Development of an Automated Quantitative Latex Immunoassay for Cardiac Troponin I in Serum,

[摘要] Currently, the measurement of troponin I (TnI) can only be accomplished through the use of heterogeneous assays on closed-system automated analyzers. The development of this new and innovative latex technology will allow the measurement of TnI on a variety of turbidimetry-based open-system instruments, greatly enhancing clinical applicability of this test.Determining the presence of TnI in the serum of patients is an important aid in the diagnosis of myocardial infarction. An advantage of TnI is its improved specificity for myocardial damage compared with creatine kinase-MB (1). In addition, there is strong evidence that future utilization of TnI will be for risk stratification to assist in the decision process for therapeutic intervention with glycoprotein II/IIIa inhibitors or low-molecular weight heparin (2)(3). In fact, the GUSTO trial, which should be completed soon, included TnI as one of the cardiac markers to be considered for risk stratification. The cardiac troponin complex is part of the contractile apparatus of the thin filament in striated muscle and consists of subunits C, T, and I. Different isoforms of TnI exist in the skeletal and cardiac muscles (fast skeletal, slow skeletal, and cardiac TnI). The distinct structural heterogeneity between these isoforms allows production of specific antibodies (4), which can be utilized by the latex assay to detect serum TnI in clinical conditions that involve myocardial damage. After …

[学科分类] 过敏症与临床免疫学