Reflectometry Interference Spectroscopy in Detection of Hepatitis B Surface Antigen

[摘要] Optical biosensors are of increasing interest for real-time detection of analytes without the use of additional labeled reagents. The sensing system is based on different interfacial physical properties that change upon the binding reaction. Combining high sensitivity and selectivity, label-free biosensors have the potential to provide low-cost detection and measurement technology for accurate and highly specific quantification of low-concentration analytes. Reflectometry interference spectroscopy (RIfS) is such a typical optical system that has been systematically developed and investigated by Brecht et al. (1). Based on the white-light interference effects occurring at thin transparent films, RIfS has shown high precision and high stability in monitoring the change of optical thickness attributable to interfacial molecular interaction and has been shown to be a useful approach for the detection of herbicides in river water and hydrocarbons in air (1)(2).At present, we are trying to find possible applications of RIfS in clinical diagnosis. ELISAs, the widely used label immunoassays in clinical laboratories, have inherent drawbacks, such as numerous pipetting and incubation steps. Additionally, the technique does not always show dose dependence in an extensive concentration range of analytes, exemplified by …

[学科分类] 过敏症与临床免疫学