Fluorogenic Probes to Detect the A−444C Transversion in the Leukotriene C4 Synthase Promoter

[摘要] Adverse hypersensitivity reactions to aspirin and other nonsteroidal antiinflammatory drugs (1) resemble pseudoallergic reactions with bronchospasm, chronic rhinoconjunctivitis, nasal polyps, urticaria, angioedema, life-threatening bronchospasm, and anaphylactic shock (2). Up to 25% of hospital admissions for acute asthma may be caused by nonsteroidal antiinflammatory drug ingestion. In aspirin-induced asthma, cysteine-leukotriene release into airways (3)(4) is associated with increased concentrations of leukotriene C4 synthase, the enzyme that forms leukotriene C4 [for review, see Ref. (5)]. A polymorphism in the promoter region of the gene for leukotriene C4 synthase predisposes to disease (4). The A−444C transversion creates a new Msp I restriction site.PCR-restriction fragment length polymorphism analysis has been used to detect this polymorphism (4). This method is time-consuming …

[学科分类] 过敏症与临床免疫学