Plasma Aldosterone Assays: Comparison between Chemiluminescence-Based and RIA Methods

[摘要] Measurement of plasma aldosterone is increasingly required in clinical practice because primary aldosteronism (PA) is the most frequent secondary cause of hypertension (1)(2)(3)(4). Chemiluminescence-based (CL) assays are attractive alternatives to traditional RIAs for hormones such as aldosterone.We compared aldosterone results obtained with a new CL method (Nichols Advantage) and an RIA [Sorin Biomedical Diagnostics (SBD)] in blood samples from 236 hypertensive patients. Blood samples were collected and immediately stored in separate aliquots at −20 °C until analysis. For 104 of the samples from these patients, we also compared the SBD-RIA results with results from a second RIA method [Maia Adaltis (MA)]. We then used the CL assay and SBD-RIA to analyze samples obtained from 27 patients before and after inhibition testing (saline loading or fludrocortisone acetate) to confirm the diagnosis of PA. In all 27 patients, the inhibition test was performed because of a previous finding of an increased aldosterone-to-renin ratio (ARR).Aldosterone was assayed in 2 aliquots from the same sample of each patient, and mean values were used …

[学科分类] 过敏症与临床免疫学