Gold-Nanoparticle-Probe–Based Assay for Rapid and Direct Detection of Mycobacterium tuberculosis DNA in Clinical Samples

[摘要] Many molecular methods have been developed for the direct identification and susceptibility testing of mycobacteria (1)(2)(3). Only 2 US Food and Drug Administration–cleared methods are available for direct detection of Mycobacterium tuberculosis , the enhanced Direct Test (Gen-Probe) and the Amplicor (Roche). The appearance of multiple-drug–resistant strains and elucidation of the rifampicin resistance mechanism in M. tuberculosis have prompted the development of new molecular techniques such as the INNO-LiPA-Rif-TB (Innogenetics), that can simultaneously detect M. tuberculosis and sensitivity/resistance to rifampicin (4). However, most molecular tests for M. tuberculosis detection are expensive and unsuitable for routine use. Therefore, we sought to develop an inexpensive molecular method for the fast and sensitive detection of M. tuberculosis .Our colorimetric method uses gold nanoparticles for rapid and sensitive direct detection of M. tuberculosis in clinical samples with high efficiency after an initial round of PCR. Gold-nanoparticle–based methods have been used extensively for detection of specific DNA and RNA sequences (5)(6)(7). We obtained 73 clinical specimens from patients with suspected pulmonary and extrapulmonary tuberculosis with …

[学科分类] 过敏症与临床免疫学