Method for Simultaneous Direct Estimation of Glucose and Xylose in Serum

[摘要] A direct procedure for measuring serum monosaccharides is presented. Borate intensifies the colors produced when these substances react with o -toluidine, which, in the case of glucose and xylose, have absorption maxima at 480 nm and 630 nm, respectively. Thus, these sugars may be determined singly or simultaneously in a single sample by differential spectrophotometry. An alternative technique in which glucose oxidase and o -toluidine are used is also presented for the estimation of xylose and other pentose sugars in the presence of glucose. These two techniques permit the measurement of a pentose and a hexose other than glucose in protein solutions containing glucose. The techniques have a high degree of correlation and reproducibility. The effects of such variables as reagent concentration, reaction time, and borate concentration on the o -toluidine reaction are presented, along with an evaluation of bilirubin and hemoglobin interference.

[学科分类] 过敏症与临床免疫学