An Improved Reagent System for the Measurement of Serum Uric Acid

[摘要] The reagent is modified as follows: Serum chromogens are destroyed by trisodium phosphate. The use of phosphotungstic acid eliminates the tungstate and sulfate anions, a frequent cause of turbidity. Triethanolamine, added to the carbonate—urea mixture, provides unusual stability of the color through its buffering action. Only 0.2 ml of serum is required. The method is highly reproducible and accurate; analytical results agree closely with those obtained by the uricase or cyanide—urea method.

[学科分类] 过敏症与临床免疫学