Free-Electrophoretic Behavior of Urea-Insulin Reaction Products

[摘要] Five reaction products, previously isolated from an aqueous mixture of insulin and concentrated urea that had been subjected to various conditions of time and temperature, were examined by moving-boundary electrophoresis. Insulin OU and insulin IU separated into major and minor peaks with mobilities identical to those observed by Timasheff et al. (and confirmed by us) for intact crystalline insulin. Insulin IIU showed a major peak (80%) with a mobility of -7.6 x 10-5 cm/s/V/cm. Insulin IIIU showed a major peak with a mobility of -9.2 x 10-5 cm/s/V/cm. On the basis of change in electron charge and the fact that guanidine does not produce the effects of urea, it is postulated that the delayed-acting urea insulin (IIU) is formed by native insulin reacting with the isocyanate ion in urea solution.

[学科分类] 过敏症与临床免疫学