Adaptation of the Zak-Epstein Automated Micromethod for Serum Iron to Determine Iron-Binding Capacity and Urinary Iron

[摘要] An automated method has been developed for determining serum iron-binding capacity and urinary iron as well as serum iron. After preliminary preparation, the sample is treated with ascorbic acid in hydrochloric acid to release and reduce protein-bound iron, then singly dialyzed into an acetate buffer and reacted with 4,7-diphenyl-1,10-phenanthroline (bathophenanthroline) to form a pink color proportionate to the amount of iron present. The sample passes through a 15-mm flow cuvet and is read at 537.5 nm, with the range expander set at either 2x or 4x.

[学科分类] 过敏症与临床免疫学