Microcalorimetric Determination of Glucose in Reference Samples of Serum

[摘要] I report a detailed series of microcalorimetric measurement of glucose concentrations in five reference samples of serum. the method utilized ( a ) calibration in the actual medium of analysis and ( b ) a correction for interferences owing to the nonspecificity of the enzyme hexokinase. The microcalorimetric results are compared with the tentative results of analyses obtained by spectrophotometric hexokinase/glucose-6-phosphate dehydrogenase method and by isotope-dilution mass spectrometry. In most cases, the microcalorimetric results appear to be in agreement with the results obtained by these latter two method. A discussion of the basis of the mocrocalorimetric measurments is presented.

[学科分类] 过敏症与临床免疫学