已收录 272903 条政策
 政策提纲
  • 暂无提纲
Double-antibody enzyme immunoassay applied to human alpha 1-fetoprotein.
[摘要] We report the development of a double-antibody system for enzyme immunoassay of human alpha 1-fetoprotein. Equilibrium and sequential saturation procedures were evaluated and compared with a similar double-antibody radioimmunoassay. With our method, 3 mug of alpha 1-fetoprotein per liter can be detected. The dose-response curve covers a 100-fold range of analyte concentrations. Within-run and between-run coefficients of variation are respectively 6.9% and 10.0%. Analytical recovery of added antigen is 99.5 +/- 10.6%. Results are routinely obtained in 24 h but can be obtained in less than 8 h. Alpha 1-fetoprotein was measured in a variety of clinical samples, including sera from 488 pregnant women. The advantages of the present method over radioimmunoassay and other enzyme immunoassay procedures are discussed.
[发布日期]  [发布机构] 
[效力级别]  [学科分类] 过敏症与临床免疫学
[关键词]  [时效性] 
   浏览次数:2      统一登录查看全文      激活码登录查看全文