Divergence in Classification of 25-Hydroxyvitamin D Status with Respect to Immunoassays

[摘要] The major problem in measuring 25-hydroxyvitamin D (25-OHD) is attributable to the molecule itself (1). Thus, an analytical method must be selected that will accurately estimate total circulating 25-OHD independent of the circulating concentrations of 25OHD2 and 25OHD3. Although HPLC remains the method of choice, many convergent data indicate that the 25-OHD RIA (DiaSorin Inc) should be incorporated as a secondary reference method (2)(3). With the RIA method actually used in our laboratory, we evaluated the 25-OHD LIAISON® chemiluminescent immunoassay (CLIA) proposed by the same manufacturer as an alternative assay. We also investigated whether a 25-OHD value <50 nmol/L, a well-known arbitrary RIA cutoff value to define vitamin D insufficiency, is applicable to CLIA.We studied 199 people [79 men/120 women; mean (SD) age 48.1 (19.8) years] who were residents of Paris and its suburbs and who suffered primary hyperparathyroidism (HPT), secondary HPT with hemodialysis or obesity, mild asthenia, depression, and/or osteopenia/osteoporosis. This study was approved by the ethics …

[学科分类] 过敏症与临床免疫学