To Mix with Pooled Normal Plasma or Not to Mix: A Comparative Study of 2 Approaches for Assessing Lupus Anticoagulant Inhibitory Activity in the Dilute Russell Viper Venom Method

[摘要] The recommended (1) step-wise approach to the study of lupus anticoagulant (LA) begins with a prolonged clotting time in a phospholipid-dependent clotting assay (screen), a noncorrection in a mix with pooled normal plasma (PNP), and then a correction in the presence of increased phospholipid (confirm). Laboratories differ in their application of these recommendations. Jacobsen et al.(2) integrate screen, mix, and confirm into a single assay and analyze the data as a lupus ratio (LR). Tripodi et al.(3) propose no prior mix with PNP even for patients on oral anticoagulant (OAC) and analyze their data as a percentage correction. We wished to compare these 2 approaches by using the common(4) dilute Russell viper venom test (dRVVT) as the clotting assay.Samples were collected from 500 consecutive patients (291 women, median age 56 years) for whom LA tests were requested during a 5-month period. Repeat tests (n = 19) were excluded on patients as were 9 …

[学科分类] 过敏症与临床免疫学