Considerably Reduced Centrifugation Time without Increased Hemolysis: Evaluation of the New BD Vacutainer® SST™II Advance

[摘要] Short in-laboratory turnaround time is important, and reducing centrifugation time can shorten this phase of the preanalytical process. The new BD Vacutainer® SST™II Advance (Becton Dickinson, article number 367955) has a semisolid, thyxotrophic acrylic gel with chemical composition identical to that of the previous SST™II tube. In the new Advance tube, however, the gel has been extended to 1 side of the tube, forming a gel nose with a larger initial contact surface that is supposed to accelerate gel movement during centrifugation to allow shorter centrifugation time (1). We compared gel barrier formation and hemolysis rates of the new Vacutainer SST™II Advance under a conventional centrifugation (CC) mode (13 min, 1700 g ; acceleration and braking time, 55 s) vs an accelerated centrifugation (AC) mode (5 min, 3000 g ; acceleration and braking time, 39 s) to determine whether this new Vacutainer system allows reduced centrifugation time while maintaining sample quality.In vitro hemolysis can be caused by mechanical stress inflicted by higher g -forces, which might artificially increase several serum analytes and cause interference in some laboratory assays (2). Therefore we measured lactate dehydrogenase …

[学科分类] 过敏症与临床免疫学