Enzymatic Kinetic Rate and End-Point Analyses of Substrate, by Use of a GeMSAEC Fast Analyzer

[摘要] Enzymatic substrate analysis is an attractive means of analysis in clinical chemistry because of its sensitivity and specificity. The GeMSAEC Fast Analyzer, in conjunction with a small computer, provides a means of performing routine enzymatic substrate analysis and offers the following advantages: ( a ) selectivity of approaches to enzymatic analysis, i.e., end-point or kinetic; ( b ) essentially parallel analyses of multiple samples, yielding a unique method for performing kinetic fixed-time analysis; ( c ) on-line data reduction, resulting in rapid calculation and output of results and the minimization of data handling errors; and ( d ) a small reagent volume per test (400 µl), which reduces the cost of analysis. The analysis of substrate with enzymatic end-point and kinetic procedures is examined by use of a computer-interfaced Fast Analyzer. Computer programs were written to facilitate this study. Glucose (hexokinase/GPD), urea (urease/GMD), and uric acid (uricase) have been used as examples in evaluating both end-point and kinetic analyses. The advantages and limitations of each type of analysis are presented, with the emphasis being placed on enzymatic substrate analysis and means by which the computer-interfaced Fast Analyzer can facilitate both end-point and kinetic analyses.

[学科分类] 过敏症与临床免疫学