Automated Hexokinase Procedure for Assaying Glucose in Urine, Serum, or Plasma

[摘要] A colorimetric hexokinase method is described for measuring glucose in urine with the AutoAnalyzer. Pretreatment of the urine is not necessary. Sampling rate is 50/h, sample requirement 0.13 ml. Absorbance is measured at 505 nm; Beer's law is followed to 300mg/liter. Results obtained with this procedure and by one in which o -toluidine was used in combination with glucose oxidase were highly correlated (r = 0.998). Glucose concentrations in serum or plasma may be determined by diluting the sample 10-fold. Bilirubin in concentrations up to 200 mg/liter do not significantly interfere. With a dilutor, 20 µl of plasma or serum is diluted to 0.2 ml directly from a capillary collection tube after centrifugation. Thus, a convenient, sensitive method is available for assaying glucose concentrations in pediatric specimens.

[学科分类] 过敏症与临床免疫学