Semiquantitation of Leucine, Isoleucine, and Valine by Thin-Layer Chromatography in Management of Maple-Syrup Urine Disease

[摘要] A few microliters of serum suffice to estimate the leucine, isoleucine, and valine present. The three branched-chain amino acids are separated by unidirectional thin-layer chromatography, and estimated by visual comparison with co-chromatographed individual standards in graduated concentrations. The method is especially applicable to the frequent monitoring of serum branchedchain amino acids needed in treating patients with maple-syrup urine disease, and may also be used for rapid diagnosis of the disease in suspected infants.

[学科分类] 过敏症与临床免疫学