Quantitation of Beta Lipoprotein (LDL) Cholesterol by Densitometric Evaluation of Disc Electropherograms

[摘要] This paper describes a rapid, facile, and accurate new procedure for determination of marginal Type IV or Type II hyperlipoproteinemias, in which discontinuous (disc) electrophoresis on polyacrylamide gel is used to quantitate LDL (beta) cholesterol without ultracentrifugation. Fasting sera, previously assayed by ultracentrifugation and chemical technique, were prestained with Sudan Black, and the lipoproteins were separated by disc electrophoresis. The beta-lipoprotein fraction of each electrophoretic pattern was quantitated by densitometry. The values for the integrated densitometric peak areas for the beta fraction and the chemically assayed cholesterol of the ultracentrifuged LDL fraction correlated well ( r = .90). Results were determined from a curve relating chemical concentration of the integrated peak values by densitometry to the known LDL cholesterol of a series of standards.

[学科分类] 过敏症与临床免疫学