Automated Vitamin C Analysis

[摘要] An automated method of analysis for vitamin C (ascorbic acid, dehydroascorbic acid, diketogulonic acid) is described, in which ascorbic acid as oxidized to dehydroascorbic acid with Chloramine-T and determined by reaction with 2,4-dinitrophenylhydrazine. Results obtained by automated and manual methods agreed within 0.89% for ascorbic acid in liver. By the automated method, 96.7% of ascorbic acid added to tissue homogenates could be accounted for. The principal drawback to the proposed method is the use of 70% perchloric acid, a potent oxidizing agent, to solubilize the osazone.

[学科分类] 过敏症与临床免疫学