An Error-Producing Interaction in an Automated Method for Measuring Cholinesterase Activity in Blood

[摘要] Blood cholinesterase activity values obtained in our laboratory by the automated procedure of Levine et al. ( Automat. Anal. Chem., Technicon Symp. 1965 ) are greater than those values obtained by a comparable manual method. This problem is caused by reaction of the standard (reduced glutathione) with the substrate (acetylthiocholine) to form acetylglutathione and thiocholine. Because thiocholine dialyzes about three times more rapidly than glutathione in the automated system and gives the same color reaction, proportionately higher activity values would be reported. Ways of correcting this problem are presented.

[学科分类] 过敏症与临床免疫学