Plasma Calcium Assay, with Use of the Jellyfish Protein, Aequorin, as a Reagent

[摘要] A previously proposed assay for plasma calcium, suitable for routine clinical measurements, has been investigated. The method is based on the calcium-sensitive luminescence of a jellyfish protein, aequorin. Advantages of this method are low cost, simplicity, and the suitability of most currently available fluorometers and spectrophotometers for the assay. Disadvantages are that aequorin may not be readily available, and that the assay apparently yields only the total and (with suitable sample preparation) the diffusible calcium concentrations.

[学科分类] 过敏症与临床免疫学