Ultraviolet Spectrophotometric Measurement of Chlordiazepoxide in Plasma

[摘要] A simple ultraviolet spectrophotometric method is described for measurement of chlordiazepoxide in blood. The drug is extracted into chloroform at pH 7.4 and, after a NaOH wash, extracted back into HCl. The drug is identified by its characteristic ultraviolet spectra at acidic and basic pH’s, and quantified from the absorbance value measured at the major acid peak of 247 nm. Although certain weakly basic drugs theoretically could interfere if present concurrently with chlordiazepoxide, we have not seen this during three years of experience. This procedure is easily combined with established methods for barbiturates and glutethimide. Specimens from more than 60 cases of documented chlordiazepoxide ingestion have had concentrations ranging from 0.2 to 6.6 mg/100 ml by this procedure.

[学科分类] 过敏症与临床免疫学