Rapid Extraction of Triglycerides from Human Adipose Tissue with Petroleum Ether

[摘要] A rapid method has been developed for extracting neutral lipid from small samples of human adipose tissue, with a petroleum ether ("Skellysolve B") as the extracting solvent. It is coupled with a simple method of transesterification, to convert the purified triglycerides to fatty acid methyl esters. Virtually no phospholipid is extracted by the petroleum ether. Analysis of the extracted lipid by gas—liquid chromatography yielded fatty acid distributions comparable to those obtained by chloroform—methanol extraction.

[学科分类] 过敏症与临床免疫学