Grafting of walnut (Juglans regia L.) with hot callusing techniques under South African conditions

[摘要] English: One of the biggest constraints to the expansion of the walnut industry in South Africa, is the availability of good quality and cost effective plant material. Since walnuts are more difficult to propagate vegetatively than most fruit and nut species, controlled conditions are required to ensure grafting success. Hot callus grafting is a bench grafting procedure subjecting grafted trees to controlled, elevated temperature and humidity, and has been implemented successfully in several countries to increase grafting success of walnut. Until now, hot callus grafting has not been investigated as a possible walnut propagation method in South Africa. The present study assessed method of heat supply and time of grafting, as important factors determining grafting success of two walnut cultivars grafted onto Juglans regia rootstocks under hot callus conditions. Although neither method of heat supply provided definite superior grafting success, localized heating of graft unions in a heated trench, gave more consistent results than heating the entire grafted tree in a heated room. Both methods seemed promising, since acceptable grafting success was obtained in some instances with both the heated trench and room. Post-grafting shoot growth on the other hand, was significantly higher for the heated trench than for the room. Both methods did, however, result in trees of a suitable size for orchard establishment after only one growing season. Results further indicated that exposure to increased temperature for a period of 25 days is more than sufficient for callus development of walnut, and very little is gained by heating grafted trees for longer. Earlier grafting, before the onset of bud break of cultivar and/or rootstock, generally resulted in improved grafting success and drastically increased shoot growth during the first growing season, providing grafted trees of saleable size in a shorter time. There were no clear differences regarding callus formation, grafting success or survival between the two cultivars evaluated, 'Chandler' and 'Serr'. As expected, more vigorous post-grafting growth was observed for 'Serr', since it is the more vigorous grower of the two. A separate hot callus grafting trial, using Paradox rootstocks, also indicated that the type of rootstock utilized in hot callus grafting may impact grafting success. More consistent results were obtained with all four cultivars grafted onto this hybrid rootstock, than with J. regia rootstocks in the preceding trial. For both J. regia and Paradox rootstocks, survival of trees through the first growing season was still unacceptably low, although results were comparable to that of other studies. Since results were available for a single season only, conclusions should be regarded as preliminary. Nevertheless, findings clearly illustrated the potential value of hot callus grafting as propagation method for walnut in South Africa and lay a foundation for the establishment of propagation guidelines using this approach.

[发布机构] University of the Free State