A Gas Chromatographic Method for the Determination of Lactic Acid in Blood

[摘要] A method for the determination of lactic acid concentration in blood by gas chroma tography is described. Lactic acid in a protein-free filtrate is oxidized by ceric sulfate to acetaldehyde. The latter compound is introduced into the gas chroma tographic column by vapor phase injection, and is quantitatively measured within 3 min. The method is specific, precise, and not affected by compounds that may interfere with the determination of lactic acid by colorimetric or enzymatic methods.A method for preserving blood samples up to 24 hr. without change in lactic acid concentration is also described.

[学科分类] 过敏症与临床免疫学