Thin-Layer Starch-Gel Electrophoresis

[摘要] A modification of the starch-gel electrophoresis technic of Smithies is presented,which enables quantitation of the resolved fractions to be carried out with little equipment other than that used for paper electrophoretic fractionation of proteins.A sheet of plastic (Stabilene film) wrapped around a kymograph type drum is evenly coated with starch-gel. The sheet is suspended in an "open strip" Durrum type cell and electrophoresis carried out. After staining with suitable stain to demonstrate proteins, lipids, or other components, the patterns are scanned in a photoelectric densitometer. Results obtained with this procedure show good reproducibility when fractionating solutions of hemoglobin and serum proteins.

[学科分类] 过敏症与临床免疫学