An Automated Colorimetric (Tetrazolium Salt) Assay for Serum Lactate Dehydrogenase

[摘要] The adaptation of a tetrazolium salt assay for serum lactate dehydrogenase to fullautomation is described. The tetrazolium salt, INT, (2-p-iodophenyl-3-p-nitrophenyl-5-phenyl tetrazolium chloride) serves as the chromogenic indicator of the enzyme activity. Phenazine methosulfate serves as an intermediate electron carrier.Estimation requires only 5 min. of incubation, and assays may be run at the rate of 60/hr. Comparison against the manual version of the assay with respect to normal and abnormal serum activity levels is excellent.

[学科分类] 过敏症与临床免疫学