Microdetermination of Protein Nitrogen with a Dumbbell Diffusion Apparatus

[摘要] The use of a two-chamber, sealed microdiffusion apparatus has been investigated for the determination of protein nitrogen on samples containing 0.5 mg. protein. The apparatus is constructed from a ground-glass ball-and-socket joint, and all operations including digestion are performed in the designed apparatus. The method has been applied to the determination of nitrogen in tobacco mosaic virus protein and found to be in good agreement with theoretical values.

[学科分类] 过敏症与临床免疫学