The Quantitation of Major 17-Ketosteroid Fractions by Gas-Liquid Chromatography

[摘要] A method for measuring the major urinary 17-ketosteroid fractions has been developed. This method includes extraction of the conjugated steroids, hydrolysis, extraction of the free steroids, purification of the extraction, and quantitation of the steroids contained in the purified extract by gas-liquid chromatography. Data are presented on the excretion of androsterone, etiocholanolone, and dehydroepiandrosterone by normal male and female subjects. The procedure is discussed with regard to accuracy, reproducibility, precision, time per analysis, and correlation with the level of total Zimmerman chromogens in the urine.

[学科分类] 过敏症与临床免疫学