Simplified Technic for Estimation of Serum Lipoprotein Cholesterol

[摘要] A phosphomolybdic acid reagent was used to identify serum alpha- and beta-lipoproteins separated electrophoretically on filter paper. Cholesterol associated with these areas on unstained strips was eluted directly into a ferrous sulfate-acetic acid mixture for colorimetric analysis. The results obtained with the proposed method compared favorably with those of Nury and Smith (7). The sums of the values obtained for the alpha- and beta-lipoprotein cholesterols agreed well with measurements of serum total cholesterol by another method (12). Sex and age differences in the concentration of alpha- and beta-lipoprotein cholesterol were suggested by results obtained on 200 randomly selected blood donors.

[学科分类] 过敏症与临床免疫学