Colorimetric Determination of Serum Isocitric Dehydrogenase

[摘要] A colorimetric assay of serum isocitric dehydrogenase has been described in which the α-ketoglutaric acid formed is determined as the hydrazone. A standard unit of lCD activity is defined as millimicromoles KG-A formed per milliliter serum per hour of incubation at 37°.Analyses of KGA and TPNH as reaction products show that 1.58 moles of KGA are formed for each mole of TPNH under the conditions of this experiment.

[学科分类] 过敏症与临床免疫学