Determination of Serum Butanol-Extractable Iodine with a Modified Alkaline Incineration Procedure

[摘要] 1. A method has been presented for the determination of serum butanol-extractable iodine. As in other methods, the serum is acidified and extracted with n -butanol, and the butanol extract is treated with Blau reagent to remove interfering compounds.2. Total iodine in the butanol is determined by a modified alkaline incineration procedure. After removal of the butanol, crystalline bovine albumin is added, and a total iodine determination is carried out.3. Careful consideration is given to the details of the procedure, including those for the construction of the standard iodide curve.

[学科分类] 过敏症与临床免疫学