Assay of Fibrinogen in Small Blood Samples

[摘要] A procedure is presented for the assay of fibrinogen in small samples of blood. A 0.2-ml. sample of whole blood is mixed in the syringe with 0.3 ml. of isotonic citrate-phosphate buffer and centrifuged in special tubes in which the cell volume is measured. From these measurements the dilution of the supernatant plasma is calculated. The plasma is assayed for fibrinogen and this result is corrected for dilution. Plasma fibrinogen values obtained in this way are comparable to those obtained with other methods that require larger samples of whole blood.

[学科分类] 过敏症与临床免疫学