Mutations in K-ras Codon 12 Detected in Plasma DNA Are Not an Indicator of Disease in Patients with Non-Small Cell Lung Cancer

[摘要] The demonstration that cell-free circulating DNA detected in the plasma of cancer patients is genetically identical to that of the primary tumor has generated substantial interest, leading to >200 publications (http://www.ncbi.nlm.nih.gov). Recently, Wang et al. (1) reported in this journal that the method chosen for DNA isolation might contribute significantly to mutation detection (in their case, K-ras mutations in the plasma of patients with colorectal cancer). Briefly, they recommended the use of a modified guanidine/Promega resin method (G/R) to isolate DNA, affirming that this method enhances assay sensitivity. We used the same approach to detect K-ras mutations in the plasma of patients with non-small cell lung cancer (NSCLC) and compared the Qiagen vs the G/R method for isolation of circulating DNA. We purified DNA from plasma samples and cancer tissues from 12 patients. The DNA in 2 aliquots of the plasma from each patient was isolated by the …

[学科分类] 过敏症与临床免疫学