Rapid, Accurate Technique for Determination of Carbon Monoxide in Blood

[摘要] We report a chromatographic technique in which a vortex method of gas extraction and a thermal conductivity detector are used to measure carbon monoxide in blood. This method has the advantages of a small sample volume (100 µl), a rapid analysis time (3 min), ease of operation, and a high correlation with results of the standard gasometric method over the entire possible range ( r = 0.999). The accuracy of this method (coefficient of variation) for measuring CO in human blood samples was 1.7%. With it, dissolved CO can be accurately measured, as demonstrated by the Bunsen solubility coefficient for plasma CO by this method of 22.0 ml of CO per liter of water per atm. (101 kN/m2) at 24 °C (theoretical value: 21.8).

[学科分类] 过敏症与临床免疫学