Measurement of Antibodies to Insulin in Serum

[摘要] I describe a method for measuring insulin antibodies in insulin-treated subjects. The antibodies are characterized by maximum binding capacity and affinity constant. Endogenous and therapeutic insulin interferes in insulin antibody assay, and it is removed by dextran—charcoal treatment at pH 3.5. Endogenous total and free insulin may be measured by a simple extension of the method.

[学科分类] 过敏症与临床免疫学