Fluorometric Analysis for N'-Formylkynurenine in Plasma and Urine

[摘要] We describe a fluorometric method of analysis for N '-formylkynurenine. The method depends on the rate of enol formation when a solution of the compound is made basic. The enol is more fluorescent than the neutral compound. The increase in fluorescence is measured, with use of 430-nm emission and 319-nm excitation wavelengths. This method is applied to the determination of N '-formylkynurenine in protein-free filtrates of plasma or urine. To our knowledge, this is the first quantitative method for determination of N '-formylkynurenine.

[学科分类] 过敏症与临床免疫学