Standardization of Serum Ceruloplasmin Concentrations in International Enzyme Units with o-Dianisidine Dihydrochloride as Substrate

[摘要] We describe a method for calculating the absorptivity (in terms of substrate consumed) of the colored solution obtained when o -dianisidine dihydrochloride is oxidized by ceruloplasmin. By oxidizing o -dianisidine dihydrochloride with known amounts of hydrogen peroxide we could determine that the molar reacting ratio of o -dianisidine to hydrogen peroxide is 2:1. So calculated, absorptivity is 9.6 ml µmol-1 cm-1, the figure used to estimate ceruloplasmin oxidase activity in terms of International Units.

[学科分类] 过敏症与临床免疫学