Separation of Free and Protein-Bound Ligand Molecules by Means of Protein-Coated Charcoal

[摘要] Separation of free and protein-bound ligand molecules by means of protein-coated charcoal has been studied in model experiments. The results can be described by applying the simple laws of mass action and dilution, and indicate the need for a careful evaluation of the charcoal that is being used and a testing against an ideal separation method whenever experimental conditions are determined or changed.

[学科分类] 过敏症与临床免疫学