New Assay for Tryptophan and Its 5-Hydroxyindole Metabolites in Blood

[摘要] We describe a new cation-exchange fluorometry procedure for separation and measurement of three 5-hydroxyindole metabolites of tryptophan in blood. The metabolites are detected and measured via the fluorescence emitted in the visible region when the separated metabolite is added to a hydrochloric acid-ascorbic acid mixture. The assay requires only 30 min and is both selective and sensitive. The normal concentration of 5-hydroxytryptamine (serotonin) in serum is 98 µg/liter; 5-hydroxyindole acetic acid and 5-hydroxytryptophan are normally present in concentrations of less than 20 µg/liter.

[学科分类] 过敏症与临床免疫学