Dual-Precipitation Method for Lipoprotein Quantitation: Improvement by Membrane Ultrafiltration of Lipemic Samples

[摘要] Plasma lipoproteins can be measured by the clinical laboratory after the major lipoprotein classes have been separated by two precipitation steps, one of which is the aggregation of very-low-density lipoproteins (VLDL) by the detergent, sodium dodecyl sulfate. Lipoprotein concentrations are expressed in terms of the cholesterol concentration within each class. However, incomplete removal of aggregated VLDL has led to serious analytical errors in a few lipemic subjects. We tested the efficacy of a membrane ultrafiltration step in removal of aggregated VLDL in three patients with plasma triglyceride concentrations of 540 to 12 560 mg/dl. Preliminary membrane ultrafiltration to remove the aggregated VLDL improved the precision of the method and is recommended for the analysis of grossly lipemic samples.

[学科分类] 过敏症与临床免疫学