Microdetermination of Chloride in Blood Plasma and Cells, by Spectrophotometric Analysis Using Solid Silver Iodate

[摘要] As an extension of the silver iodate method of Sendroy, a convenient, reproducible, and sensitive method for analysis of chloride in 0.02-ml. samples of serum, whole blood, or red cells is described. The procedure involves (1) preparation of a protein-free filtrate of the sample, (2) Agl03 reaction with the filtrate, and (3) spectrophotometric analysis of iodine as the quantitative measure of the amount of iodate exchanged for chloride. Conditions important for each of these steps have been investigated. A precision of ± 0.85 (S.D.) mM CI per liter has been found for this method, the results of which are within 1% of those obtained by the macro nitric acid digestion method of Van Slyke.

[学科分类] 过敏症与临床免疫学