Extraction Procedure for the Measurement of Blood Volume with Brilliant Vital Red

[摘要] A simple extraction method usable for Brilliant Vital Red is described. The dye is displaced from its combination with albumin by detergent, adsorbed on a cotton cellulose column, and eluted with 40% acetone-water at pH 8.0. Recovery of Brilliant Vital Red from plasma averaged 95%. Measurements are not affected when the plasma samples are hemolyzed, jaundiced, or lipemic, or when dextran is present. Patients therefore require no previous preparation. Simultaneous measurements of blood volume were made by Brilliant Vital Red and l131-albumin in 17 patients. The results of the two methods were in close agreement.

[学科分类] 过敏症与临床免疫学