Photometric Microdetermination of Serum Lipase with a Phenyl Laurate Substrate
[摘要] The Raderecht and Moskau procedure for pancreatic lipase (6) has been modified for determining the small amounts of this enzyme present in normal blood serum. The present investigation confirmed their findings: that phenyl laurate provides a stable easily-prepared substrate of uniform composition; that the substrate is specifically split by serum and pancreatic lipase; and that the reaction product (phenol) can be readily determined by a simple and sensitive colorimetric procedure.The lipase units are expressed in the same manner as are phosphatase units; i.e., milligrams of phenol liberated per 100 ml. serum at 370° in 30 min. The normal range, based on 40 sera, was found to be 2.5-5.6 units.The serum lipase values were shown to be significantly elevated in a number of cases with acute pancreatic disease. The advantage of this procedure over others presently employed for diagnostic evaluation of these conditions is discussed.
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[效力级别] [学科分类] 过敏症与临床免疫学
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