Simplified Technic for the Determination of Serum Protein-Bound Iodine

[摘要] A simplified technic for the determination of serum protein-bound iodine (PBI) is presented. Endogenously labeled l131 PBI is completely recovered. The technic is based on the method of Barker et al. (1) and includes the following modifications: The number of washes of the precipitated serum proteins are decreased. Unashed iodide standards are used in a sodium carbonate solution rather than an internal standard. A temperature-correction formula is employed to allow the cericarsenite-iodide reaction to be carried out at room temperature, and a stable cerate color is produced with brucine sulfate so that the colorimetry is carried out with greater convenience. The serum PBI concentrations are read directly from a graph, which simplifies the calculations.

[学科分类] 过敏症与临床免疫学