Sulfur Content of Paper Electrophoretic Fractions In Human Serum

[摘要] Instrumentation is described for scanning paper electrophoretic strips for the sulfur distribution of the fractionated serum proteins with the vacuum X-ray spectrometer. Paper electrophoretic patterns of serum proteins scanned by X-ray spectrometry for sulfur content of the various fractions and by protein staining are compared.For 26 blood donors, the mean percentages of sulfur found in the different fractions were: albumin, 1.64; and globulins: alpha1, 0.63; alpha2, 1.10; beta, 1.14; and gamma, 0.98. The procedure was then applied to the serum of patients with diseases of the liver and of the kidney, various types of malignancies, and other diseases.The percentage of sulfur in abnormal fractions varies widely. This permits, in some cases, detection of abnormal peaks which would otherwise be missed by protein staining alone.This technic should be of value in the identification and classification of abnormal protein fractions, when combined with studies by the ultracentrifuge and immuno-electrophoresis.

[学科分类] 过敏症与临床免疫学