A Source of Error in an AutoAnalyzer Determination of Serum Iron

[摘要] An AutoAnalyzer method for serum iron was found to be giving erroneously high values owing to a Donnan equilibrium effect on the rate of dialysis of ferrous ions. The addition of neutral salt to the reagent corrected the deficiency. The validity of other assays on the AutoAnalyzer that measure dialyzable ions in the presence of relatively high concentrations of protein should be verified in view of this finding.

[学科分类] 过敏症与临床免疫学