A Modified Copper Method for the Estimation of α-Amino Nitrogen in Urine

[摘要] The copper method of Pope and Stevens (1) for estimation of α-amino N has been modified by a change in buffer and the use of tetraethylenepentamine as a color intensifier, giving a good colorimetric assay. α-Amino N was measured successfully in concentrations of 0.2-2.8 mg./10 ml. Interference due to ammonia has been minimized, and interference due to certain other materials has been determined with suggestions for its elimination. The modified procedure offers the advantages of simplicity and speed in obtaining an approximate determination of α-amino N in urine.

[学科分类] 过敏症与临床免疫学