Improved measurement of acetylsalicylic acid esterase in serum.

[摘要] A fixed-time incubation method for measuring acetylsalicylic acid esterase was improved by using a higher concentration of the substrate, acetylsalicylic acid, and an activator, Ca2+. The enzymatic activity in serum was 25-fold that measured by earlier methods. Inhibition studies showed a pattern similar to that reported for cholinesterase. Use of cholinesterase inhibitor in sample tubes for determination of acetylsalicylic acid and salicylic acid is recommended.

[学科分类] 过敏症与临床免疫学