Automated rate-immunonephelometric determination of serum prealbumin (transthyretin).

[摘要] We describe an automated determination of serum prealbumin (transthyretin), based on the maximum rate of light scatter of an antigen-antibody precipitate. Optimal dilutions of antibody reagent and of 100-microL serum samples are given. Within- and between-assay CVs are less than 5%. Results by this method are nearly identical with those obtained by a radial-immunodiffusion technique. Measuring the rate of light scatter provides a considerably faster test than equilibrium scatter, electrophoretic, or radial-immunodiffusion methods. Treatment before analysis to clear lipemic sera obviates falsely high results.

[学科分类] 过敏症与临床免疫学